Sample Preparation and Isolation of RNA or DNA
Freshly Frozen and RNAlater®/RNARetain™-treated Tissues
Asuragen routinely isolates nucleic acid from a wide variety of sample types including
plant tissue, human and other animal tissues, cell lines, blood, and bacteria. In
addition, we have significant experience isolating nucleic acids from tissues that
have undergone a variety of preservation processes including fresh, frozen, RNAlater-treated,
OCT-embedded, and formalin fixed paraffin embedded (FFPE)
. Learn more about
how we qualify our methods and staff.
Not all tissues are equal. Consult with our experts to discuss your tissue types,
tissue processing, and any downstream applications to obtain a better understanding
of the amount of tissues required for your experimental design.
Description of Services
Features of these sample prep services:
- RNA isolation methods optimized to recover miRNA
- Asuragen-isolated RNA or DNA suitable for numerous downstream
applications, including expression profiling and genotyping studies (additional
services)
- Basic histology services also available
Workflow for sample prep:
- Tissue disruption
- Organic extraction-based isolation and cleanup
- DNAse treatment (if applicable)
- QC by NanoDrop spectrophotometer and Agilent 2100 Bioanalyzer
- Re-purification and/or concentration if required
Deliverables
Regardless of downstream application, detailed QC reports including nucleic acid
concentration, RNA integrity (RIN) and electropherograms (where applicable), and purity
are provided on each sample. Purified nucleic acid samples are shipped back to you
on dry ice. Asuragen ships samples Monday through Wednesday and will notify you
upon shipment.
Isolation services are often utilized with Asuragen’s
gene expression services,
miRNA profiling services, or
genotyping services. Surplus RNA or DNA will be discarded unless
otherwise requested. Additional shipping charges may apply for the return of residual
blocks, RNA, or DNA.
Quality Control
No matter how robust the downstream processes, good data is dependent on sample
quality; consequently, accurate measurement of sample quality is essential before
deciding if a sample merits further analysis.
For RNA
All samples prepared by Asuragen Services are routinely qualified by spectrophotometry
with the NanoDrop spectrophotometer
and the Agilent 2100 Bioanalyzer. If you have chosen to use Asuragen
Services for additional services and a sample does not pass the recommended QC metrics
for analysis, you will be contacted promptly to discuss possible solutions. These
may include replacing the sample or re-isolating the RNA, excluding the sample from
further analysis, or proceeding at-risk.
High Quality RNA
RNA was isolated from colon tumor tissue by Asuragen and run on the Agilent 2100
BioAnalyzer to show integrity (red line). The same RNA was incubated overnight at
37°C to show stability (blue line). This data shows that there was no change in
RNA quality and integrity after extended incubation.
For DNA
All DNA samples prepared by Asuragen Services are routinely qualified by spectrophotometry
with the NanoDrop spectrophotometer
and gel electrophoresis. Due to the degraded nature of DNA from FFPE tissues, special
quality control measurements are used.
These rigorous procedures enable Asuragen to be the only service provider to offer
a Data Quality Guarantee:
If, for any reason, a qualified sample does not pass our process control specifications,
we will re-run the sample at no additional charge.
Read more about our
commitment to the best possible data quality, or read about our contributions to
in-process controls and
standardization.
Turn-around Time
Most projects are completed in one to two weeks. If you have a critical time requirement,
we can often meet your need for no additional charge.
Read more about our commitment to prompt turn-around times.
Typical Yields
Human Tissue Standard Yields*
Rodent Tissue Standard Yields*
* Yield information derived from large scale preps; these values should be used
as a nominal guide. Please consult with our technical experts prior to submitting
samples.
Additional Services Available
mRNA Analysis:
Affymetrix Gene Expression Profiling Service
Illumina Gene Expression Profiling Services
Applied
Biosystems TaqMan Gene Expression Services
miRNA Analysis:
Ambion/Affymetrix
DiscovArray miRNA Expression Profiling
Agilent miRNA Microarray Service
Applied
Biosystems TaqMan miRNA Expression Services
DNA Analysis:
Agilent aCGH
Affymetrix SNP 6.0 - coming soon
Data Analysis:
Asuragen’s Informatics
Team offers a broad range of analytical services to help you get the most information
from your data.
Catalog Numbers for Frozen/RNAlater/RNARetain-treated
Tissues Sample Prep Services
|
CA0009
|
Isolation, Total RNA from fresh tissue
|
|
CA0025
|
Isolation, DNA
|
Requirements for Frozen/RNAlater/RNARetain-treated Tissues Sample Prep and Related
Services
Sample Input Requirements for the services listed above are found in the Sample Submission Form and Shipping Instructions.